Non disponible en dehors du Royaume-Uni et de l'Irlande
Application
BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.
2-mercaptoethanol is widely used for retarding oxidation of biological compounds in solution.
General description
2-mercaptoethanol is a thiol compound, commonly used as a reducing agent in organic reactions.
Packaging
1, 2.5 L in glass bottle
10, 100, 250, 500 mL in glass bottle
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