Non disponible en dehors du Royaume-Uni et de l'Irlande

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SH (B3657).

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 50 mM KCl, 10 mM 2-mercaptoethanol, 0.1% gelatine (v/v), 0.01% polydocanol (v/v), 50% glycerol (v/v) at 4 °C

Specificity

Recognition sequence: 5′-C/C(A,T)(T,A)GG-3′Ligation and recutting results: After 2-10-fold Sty I overdigestion of 1 µg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.Heat inactivation: Inactivated at 65 °C for 15 minutes.