Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Pigment epithelium-derived factor (PEDF) has been used in cell culture for the production of pericytes and in Western blotting.

Biochem/physiol Actions

Pigment epithelium-derived factor (PEDF) has neurotrophic, anti-angiogenic and anti-tumorigenic properties. A key component of the anti-angiogenic action of PEDF is the induction of apoptosis in proliferating endothelial cells. Additionally, it is capable of inhibiting the activity of angiogenic factors such as vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF-2). The recognition of a lipase-linked cell membrane receptor for PEDF (PEDF-R) that binds to PEDF with high affinity should facilitate further elucidation of the underlying mechanisms of this pluripotent serpin. The unique range of PEDF activities associate it as a potential therapeutic agent for the treatment of vasculature related neurodegenerative diseases such as age-related macular degeneration (AMD) and proliferative diabetic retinopathy (PDR). PEDF in addition has the potential to be functional in the treatment of various angiogenesis-related diseases including a number of cancers.

General description

Pigment epithelium-derived factor (PEDF) is a non-inhibitory serpin. It is a 50,000Da glycoprotein created and secreted in many tissues all the way through the body. The gene encoding this protein is localized on human chromosome 17p13.3. Recombinant human PEDF produced in Escherichia coli is a single, non-glycosylated, polypeptide chain containing 400 amino acids and having a molecular mass of 44.5kDa.

Physical form

The sterile filtered, concentrated (1 mg/ml) protein solution was lyophilized from 20 mM PBS and 150 mM NaCl, pH-7.4.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in sterile dH₂O to a concentration of 0.1 -1 mg/mL and let the lyophilized pellet dissolve completely. This solution can then be diluted into other aqueous buffers.