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Biochem/physiol Actions

ANGPTL-3 (Angiopoietin like protein 3) can exert activities related to both angiogenesis and lipid metabolism. ANGPTL-3 inhibits lipoprotein lipase (LPL) and endothelial lipase (EL), which has the effect of increasing plasma levels of triglycerides and HDL (high-density lipoprotein)-associated cholesterol. The fibrinogen like portion of the ANGPTL-3 protein can bind α-5/β-3 integrins leading to endothelial cell adhesion and migration. Mutations in this gene can result in familial combined hypobetalipoproteinemia.

General description

ANGPTL-3 (Angiopoietin like protein 3) is a member of the angiopoietin family of structurally related proteins, characterized by a coiled N-terminal domain and a C-terminal fibrinogen like domain. It is mainly present in the hepatocytes and is also secreted into circulation. The gene is mapped to human chromosome 1p31.Recombinant human ANGPTL-3 is a glycoprotein that migrates by SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) analysis at an apparent molecular weight of 62kDa, and contains 452 amino acid residues including a C-terminal His tag.

Physical form

Lyophilized from 10mM Sodium Phosphate, pH 7.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.