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Biochem/physiol Actions

Hepatocyte growth factor (HGF) functions via its receptor c-Met, which gets activated upon tyrosine phosphorylation, and subsequently promotes epithelial cell proliferation, morphogenesis, angiogenesis and motility. It plays a crucial role in organ development, as inactivation of either this gene or its receptor in fetal stages results in organ hypoplasia. Endogenously this protein is involved in the self-repair of various ograns including injured livers, kidneys, lungs etc. HGF is a predominant potent factor involved in lung growth and repair. It functions as a motogen, tubulogen and anti-apoptotic agent on airway epithelial and other cells expressing HGF receptor c-Met. Studies in mice show that HGF determines the severity of Pseudomonas aeruginosa keratitis by enhanced HGF signaling through c-met resulting in elevated pro-inflammatory cytokines and reduced anti-inflammatory cytokines.

General description

Hepatocyte growth factor (HGF) is a plasmin related disulfide-linked, hetereodimeric protein containing an α and a β chain. It is synthesized as inactive, single-chain precursor which shares similarity with plasminogen. Its α chain contains an N-terminal hairpin and four kringle domains (NK4).

Physical form

Lyophilized from 10 mM Tris, pH 7.2 + 100mM L-Arginine + 200 mM NaCl.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Note: Slow to dissolve. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.