Non disponible en dehors du Royaume-Uni et de l'Irlande

Biochem/physiol Actions

Prokineticin-2 (PK2) plays a vital role in regulation of various biological functions, such as gastrointestinal motility, angiogenesis and circadian rhythms. It is closely related to EG-VEGF (endocrine gland-derived vascular endothelial growth factor)/prokineticin-1 (PK1). PK1 and PK2 exert their function by interacting with two G protein-coupled receptors PKR1 and PKR2, respectively. PK2 is a candidate gene for the pathogenesis of mood disorders.

General description

Prokineticin-2 (PK2) is a cysteine-rich secreted protein that is expressed in the testis and in lower levels of the small intestine. PK2 regulates various biological functions including gastrointestinal motility, angiogenesis and circadiam rhythms. It is closely related to EG-VEGF (Prokineticin-1) and binds to two orphan B-protein-coupled receptors termed PK-R1 and PK-R2. Recombinant human Prokineticin-2 is an 8.8 kDa protein consisting of 81 amino acid residues and ten cysteine residues that potentially form five intra-molecular disulfide bonds.It also contains completely conserved N-terminal hexapeptide sequence that is involved in biological activity. PK2 is encoded by the gene mapped to human chromosome 3p13.

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Sequence

AVITGACDKD SQCGGGMCCA VSIWVKSIRI CTPMGKLGDS CHPLTRKVPF FGRRMHHTCP CLPGLACLRT SFNRFICLAQ K