Protéine Kinase CbetaI isozyme humain, > = 95 % (SDS- PAGE), recombinant ,exprimé en cellules D'insectes infectés par le baculovirus, solution aqueuse tamponnée de glycérol

Code: p1787-20ug D2-231

Non disponible en dehors du Royaume-Uni et de l'Irlande

Biochem/physiol Actions

Protein Kinase C (PKC) is a serine/threonine kinase that is activated intracellularly by signal transduction pathways that produce DAG from phosphatid...


en savoir plus

Votre prix
£968.00 20UG

Non disponible en dehors du Royaume-Uni et de l'Irlande

Biochem/physiol Actions

Protein Kinase C (PKC) is a serine/threonine kinase that is activated intracellularly by signal transduction pathways that produce DAG from phosphatidylinositol diphosphate (PIP2) and phosphatidylcholine (PC) through the action of various activated phospholipases. Phorbol esters also stimulate PKC. At least 11 PKC isozymes have been identified that differ in primary structure, tissue distribution, subcellular localization, response to extracellular signals, and substrate specificity. The isozymes can be grouped into three subfamilies. Members of the first family require Ca2+ and phospholipid and include PKCα, βI, βII, and γ. Members of the second family are phospholipid-dependent but Ca2+-independent, and include PKCδ, ε, η, and θ. Members of the third family are not activated by either DAG or phorbol esters and include PKCξ, µ, and ι.Phosphorylation appears to be an important mechanism of regulation of all PKCs. PKC plays a role in the regulation of cell transformation, growth, differentiation, ruffling, vesicle trafficking, apoptosis and gene expression.

Physical form

Solution in 20 mM HEPES, pH 7.4; 2 mM EDTA, 2 mM EGTA, 5 mM DTT, 100 mM NaCl, 0.05% Triton X-100, and 50% glycerol.

Unit Definition

One unit will transfer 1 nmol of phosphate to histone H1 in 1 min at pH 7.4 at 30 °C.

assay≥95% (SDS-PAGE)
formbuffered aqueous glycerol solution
Gene Informationhuman ... PRKCB(5579)
mol wtapparent mol wt 79-80 kDa
Quality Level200
recombinantexpressed in baculovirus infected insect cells
shipped indry ice
specific activity≥700 units/mg protein
storage temp.−70°C
UniProt accession no.P05771
Ce produit répond aux critères suivants: