Non disponible en dehors du Royaume-Uni et de l'Irlande

Biochem/physiol Actions

Catalyzes the phosphorylation of the terminal tyrosine residues of the Tec protein, a downstream protein in intracellular signaling pathways.

Other Notes

Histidine tagged.

Physical form

Solution in 50 mM Tris, pH 7.5, 0.05 mM EDTA, 1 mM DTT, 100 mM NaCl, 0.05% NP-40, and 50% glycerol.

Unit Definition

Using 50 mM HEPES, pH 7.5, 10 mM MgCl₂, 2.5 mM DTT, 10% glycerol, 0.01% Triton X-100, 200 µM ATP, 200 µg/mL poly [Glu, Tyr] 4:1 and trace [32P]-gamma ATP at 30°C