Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

The biological activity is measured by its ability to induce proliferation of leptin-dependent human OB-R transfected murine BAF3 cells.

Application

Leptin from mouse has been used:to check the effect of leptin on ovulation for treating infertility in obese miceto check the effect of leptin on MGARP (mitochondria-localized glutamic acid-rich protein) expression in testis and follicular granular cells of miceto study the morphometrical changes in mouse testicular tissue upon long term exposure to leptin

Biochem/physiol Actions

Absence of leptin in mice causes severe obesity and hypothermia at temperature below thermoneutrality. It also controls feeding behavior. Leptin signaling is crucial for the bone formation.

Leptin is a hormone produced primarily in adipocytes, although leptin mRNA has also been identified in placenta and fetal tissues, gastric tissue and liver. Its primary site of action appears to be on neurons in the hypothalamus that are involved in regulating energy balance, appetite, and body weight. Leptin increases the production of nitric oxide in endothelial cells and stimulates angiogenesis in vitro and in vivo.Human and mouse leptin share ~84% sequence identity.

Physical form

Lyophilized from a 0.2 µm filtered solution with 0.0045 mM NaHCO₃