Non disponible en dehors du Royaume-Uni et de l'Irlande

Biochem/physiol Actions

Studies have indicated that DnaJ heat shock protein family (Hsp40) member C8 (DNAJC8) represses the aggregation of polyQ-containing protein in a model of Machado-Joseph disease, also referred to as spinocerebellar ataxia type 3 (SCA3). Hsp40 has its primary function to induce ATPase (adenosine triphosphatase) action of chaperones Hsp70s. Hsp40 protein is also associated with the translation and structural modification of proteins, mediating its folding and unfolding. It is also involved in protein translocation and degradation. Overexpression of HSP40 reduces aggregate formation and suppresses apoptosis in a neuronal cell model of spinal and bulbar muscular atrophy (inherited neurodegenerative diseases caused by polyglutamine expansion).

General description

Heat shock protein 40 (HSP40) is synthesized in cells in response to heat shock and other metabolic stresses and provide a transient state of thermotolerance. HSP40 is localized faintly throughout the cell in non-heat-shocked cells and accumulates in the nuclei and nucleoli in heat-shocked cells. The intracellular localization of HSP40 is very similar to that of HSP70.The DNAJC8 (DnaJ heat shock protein family (Hsp40) member C8) gene is located on human chromosome 1p35.3. The protein is predominantly expressed in human lens epithelial (HLE) cells.

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles