Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

Protein determined by biuret.

Application

Phospholipase A2 from honey bee venom (Apis mellifera) has been usedto determine renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenationin the treatment of photosystem-II (PSII) dimer complex of Synechocystis to study changes in oxygen-evolving activityas a standard in phospholipase activity assay

Biochem/physiol Actions

Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.

General description

Phospholipase A2 from honey bee venom (Apis mellifera) is a calcium-dependent lipolytic enzyme.

Packaging

1, 5, 25 mg in glass bottle

Unit Definition

One unit will hydrolyze 1.0 µmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.9 at 25 °C.