Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

The biological activity is measured by its ability to stimulate proliferation of NR6R-3T3 fibroblasts.

Application

Fibroblast Growth Factor-8b from mouse has been used-for subgerminal cavity injection in fertilized quail, chicken, turtle and emu eggsin the induction of neural differentiation in stem cells from Human Exfoliated Deciduous teeth (SHED) andin experiments conducted on fertilized hens′ eggs, where FGF8 induced SOX3 gene.

Biochem/physiol Actions

FGF-8b is involved in ectodermal differentiation in the post-gastrulation embryo, including outgrowth and patterning of the face, limbs, and central nervous system. FGF-8b activates the "c" splice form of FGFR-2, FGFR-3, and FGFR-4.

Fibroblast growth factor 8b (FGF8b) isoform of FGF8 has the most potent angiogenic and tumorigenic properties. In prostate cancer, it is responsible for angiogenesis, tumorigenesis and invasion. Exogenous expression of this gene in prostate epithelium leads to prostatic intraepithelial neoplasia (PIN). It also interacts with pattern recognition receptor long pentraxin-3 (PTX3), which is involved in local inflammation.

General description

Fibroblast growth factor 8 (FGF8) is a member of the FGF family, and was initially cloned from androgen-dependent mouse mammary carcinoma cell line. Alternative splicing of this gene produces four different isoforms mainly, FGF8a, FGF8b, FGF8e and FGF8f.

Packaging

25 µg in glass bottle

Physical form

Lyophilized from a 0.2µm filtered solution in 20mM MOPS, 0.1M sodium sulfate, 0.02% Brij-35, pH 7 containing 50ug of BSA per 1µg as a carrier protein