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Analysis Note

The activity of human angiogenin is tested in culture by measuring its ribonucleolytic activity for yeast tRNA.

Application

Angiogenin human has been used to treat chicken eggs to determine its activity by chorioallantoic membrane (CAM) analysis . It has also been used to determine the angiogenic activity of Candida albicans and Streptococcus pneumoniae cultures by chicken chorioallantoic membrane assay .

Biochem/physiol Actions

Angiogenin, a liver-derived angiogenic factor, is a single chain polypeptide with an isoelectric point of pl >9.5 and a member of the ribonuclease superfamily. Although the ribonucleolytic activity of angiogenin toward most RNase A substrates is lower than that of RNase A, this activity is essential to its angiogenic activity since inhibition of the angiogenin RNase activity will inhibit the angiogenic activity. High affinity receptors for angiogenin are expressed in cultured aortic smooth muscle cells and can be modulated by protamin, heparin and placental ribonuclease inhibitor.

Angiogenin, a liver-derived angiogenic factor, is a single chain polypeptide with an isoelectric point of pl >9.5 and a member of the ribonuclease superfamily.

Angiogenin plays a role in several biological functions such as tumorigenesis, innate immunity, neuroprotection. It also plays a role in reproduction, regeneration of damaged tissues and neovascularization.

General description

Angiogenin is a secreted growth factor. It is localized in the tissue fluids such as plasma, amniotic fluid, cerebrospinal fluid and tumor microenvironment.

Physical form

Lyophilized from a 0.2 µm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.