Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

This product is intended for use with the components of the TRAPEZE Telomerase Detection Kit, the TRAPEZE XL Telomerase Detection Kit & the TRAPEZE ELISA Telomerase Detection Kit.

Instructions for Use

1. Resuspend the cell pellet in 200 µl of 1X CHAPS Lysis Buffer.

2. Incubate the suspension on ice for 30 minutes.

3. Spin the sample in a microcentrifuge at 12,000 X g for 20 minutes at 4°C.

4. Transfer 160 µl of the supernatant into a fresh tube.

5. Aliquot and quick-freeze the remaining extract on dry ice, and store at -75°C to -85°C. The extracts for the TRAP assay should be quick-frozen on dry ice after each use. Aliquots should not be freeze-thawed more than 5 times to avoid loss of telomerase activity. Additionally, aliquoting reduces the risk of contamination.

6. Use 1000 cell equivalents (2 µl of a 1:10 dilution) per TRAP assay, according to the kit manuals.

Research CategoryAll

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

This product is intended for use with the components of the TRAPEZE^™ Telomerase Detection Kit (Catalog # S7700), the TRAPEZE™ XL Telomerase Detection Kit (Catalog # S7707) and the TRAPEZE™ ELISA Telomerase Detection Kit (Catalog # S7750). Recommended Taq polymerases: must be non-proofreading, having no exonuclease activity and capable of "hot-start." Titanium Taq™, Platinum™ Taq are suggested.



Material Provided

Each vial of S7701 TRAPEZE™ Positive Control Cell Pellet contains one million cells, pelletted to the bottom of the microcentrifuge tube.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

TRAPEZE is a trademark of Merck KGaA, Darmstadt, Germany

Packaging

1 vial in 1x10(e6) cells

1x10(e6) cells

Storage and Stability

Must be stored at -75°C to -85°C. Telomerase in specimens stored as a cell pellet is stable at this temperature for several years. Once the cell extract is made (in 1X CHAPS Lysis Buffer), it is stable for 1 year, if not subjected to more than 5 freeze-thaw cycles