Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

For research use only. Not for use in diagnostic procedures.Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Signal transducer and activator of transcription 1 (STAT1) undergoes phosphorylation on serine-727. This Phospho-Stat1 regulates pro- and anti-apoptotic genes following several stress-induced responses. STAT-1 interacts with p53 and promotes p53-mediated transcriptional gene activity and apoptosis. STAT-1 is an essential factor of stress-induced and DNA damage-induced checkpoint pathways and thus, acts as a tumour suppressor. STAT-1 functions as a pro-apoptotic factor in cardiac myocytes, brain or liver exposed to ischaemia–reperfusion injury. Thus, inhibition of STAT-1-activated pathway by epigallocatechin-3-gallate (EGCG), can be considered as a potential therapeutic method against ischaemic tissue injury. Repression of STAT-1 expression by human papillomavirus (HPV) oncoproteins E6 and E7 at transcription level is essential for genome amplification and maintenance of episomes, which is suggested to have a crucial role in viral pathogenesis.

General description

The Phospho-Stat1 (pSer⁷²⁷) ELISA (Enzyme-Linked Immunosorbent Assay) kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates.

Immunogen

Stat1 (pTyr⁷²⁷) synthetic phosphopeptide, Recombinant Human STAT1