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Analysis Note

Use 125 µl CelLytic M for 10⁶-10⁷ of suspended cells. For adherent cells, use 500-1,000 µL for a 100 mm plate; 200-400 µL for a 35 mm plate.

Features and Benefits

Efficient: Up to 50% more efficient than freeze thaw, sonication and other products Non-denaturing: Does not interfere in downstream applications such immunoprecipitation, kinase and phosphatase assays, reporter gene assays and gel shift assays Convenient: Ready-to-use reagent requires no scraping from culture plates Fast: Rapid cell lysis at room temperature.

General description

CelLytic M is a proprietary detergent solution designed for efficient whole-cell protein extraction from cultured mammalian cells. It enables efficient and rapid cell lysis and solubilization of proteins for both suspension and adherent cells. Lysates can be used in many downstream applications without removing the CelLytic M such as reporter gene assays, Western blots/immunoprecipitation, electrophoretic mobility shift assays, phosphatase assays and kinase asssays.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Quantity

CelLytic M reagent efficiency for protein extraction has been tested on, but not limited to, HeLa, CHO, COS, HL-60, Jurkat, A431, PC-12, and Bovine Aorta Endothelial Cells (BAEC).