Non disponible en dehors du Royaume-Uni et de l'Irlande
Application
This enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid whencoupled with the related enzymes in clinical analysis.For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid.
Used in the Sialic Acid Quantification Kit, SIALIC-Q
Packaging
25 units in glass bottle
Physical form
Lyophilized powder containing mannitol and EDTA
Physical properties
Isoelectric point: 4.6 ± 0.1Michaelis constant: 2.5 x 10‾3M (N-Acetylneuraminic acid)Structure: 3 subunits (approx. 35,000) per mol of enzymeInhibitors: p-Chloromercuribenzoate, sodium dodecyl sulfact, Hg++, Ag+ Optimum pH: 7.5– 8.0Optimum temp: 70°CpH Stability: pH 6.0–9.0 (10°C, 25hr)Thermal stability: Below 65°C (pH 7.5, 30 min)
Unit Definition
One unit will release 1.0 µmole of pyruvate from NANA per min at pH 7.7 at 37 °C.
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