Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

To strongly suppress more interferon responses than single B18R to enable more expression of a Simplicon™ RNA or regular mRNA(s).

B18R-E3L Plasmid (human codon optimized for B18R and E3L) is provided as a polycistronic DNA with both the B18R and E3L genes to more strongly suppress the interferon (IFN) responses that degrades Simplicon™ RNA(s) or regular mRNA(s). Upon transcription, B18R RNA (E3L) works at single co-transfection with Simplicon™ RNA or repeated co-transfection with regular mRNA(s). B18R RNA (E3L) can also be used for production of B18R-CM (B18R-conditioned medium).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Simplicon™ is a novel system to effect immediate high sustained protein expression of the target gene(s) without the risk of integration. The technology employs a self-replicating RNA based on the Venezuelan equine encephalitis (VEE) genome. By nature of a synthetic and self-replicative RNA, interferon (IFN) responses occur at the transfection and replication of Simplicon™ RNA. The IFN responses reduce the expression and lifetime of Simplicon™ RNA. Therefore, it is important to suppress the IFN responses in Simplicon™ System. B18R acts as a decoy receptor for Type I Interferons and inhibit IFN-alpha, beta, and omega and other Type I IFN receptors. When only employing B18R, some IFN-beta response still occurs. The vaccinia virus E3L gene encodes two double-stranded RNA binding proteins that suppressed IFN-beta response and improved the expression of Simplicon™ RNA at RNA transfection and continuous cell culture. In product B18R-E3L plasmid (human codon optimized for B18R and E3L), B18R and E3L are provided as a polycistronic DNA that can be transcribed into a polycistronic mRNA to be co-transfected with Simplicon™ RNA(s) for IFN suppression. For continuous expression, B18R protein is needed to supply with B18R-conditioned medium (B18R-CM) or recombinant protein.

Legal Information

SIMPLICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to lot specific datasheet.

Storage and Stability

B18R-E3L Plasmid (Cat. # SCR727). One (1) vial containing 10 µL of Plasmid (1 µg/µL). Store at -20 °C