Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

Acetylcholinesterase is measured according to Ellman, et al.

Application

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used:in the hydrolysis of substrates p-nitrophenylphosphorylcholine (p-NPPC) and p-nitrophenylphosphorylphosphate (p-NPP)to cleave glycosylphosphatidylinositol (GPI) anchor of lynx1 protein and its detachment from plasma membraneto cleave immunolabeled HeLa cells

Biochem/physiol Actions

Phospholipase C (PI-PLC) releases diacylglycerol by cleaving the glycosylphosphatidylinositol. It has broad substrate specificity and its activity is influenced by metal ions and surfactants.

Used for the release of GPI anchored proteins from the membrane.

General description

Phospholipase C (PI-PLC) from Bacillus cereus has irregular triosephosphate isomerase (TIM)-barrel structure with eight-standard parallel β barrel. It is a 28 kDa protein.

Packaging

5, 25 units in poly bottle

Physical form

Solution in 60% (v/v) glycerol containing 10 mM Tris-HCl, pH 8.0 and 10 mM EDTA

Unit Definition

One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).