Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

The Mitochondria Isolation Kit provides a fast and easy isolation of an enriched mitochondrial fraction from animal tissues as well as the testing of the electrochemical proton gradient (δψ) of the inner mitochondrial membrane. Useful for mitochondria mediated apoptosis studies.

Mitochondria Isolation Kit has been used:for the separation of mitochondrial and cytosolic fraction for determining the branched-chain aminotransferase (BCAT) activityfor the isolation of mitochondria from glomeruli and heartfor the isolation of mitochondria from injured brain samples for the measurement of mitochondrial transmembrane potential

Features and Benefits

Specially formulated extraction reagents & proven procedure suitable for small scale isolation - Isolate an enriched, intact mitochodrial fraction in a microfuge tube. Produces functionally active, intact mitochondria - Resulting mitochondria are suitable for in vitro assays for apoptosis, oxidative stress or other studies. Includes stain & protocol for determining inner membrane integrity - Determine the integrity of the inner mitochondrial membrane without the need to purchase other reagents. Compatible with the Cytochrome c Oxidase Assay Kit - Allows easy determination of the integrity of the outer mitochondrial membrane