Non disponible en dehors du Royaume-Uni et de l'Irlande
Analysis Note
Agglutination activity is expressed in µg/ml and is determined from serial dilution in phosphate buffered saline, pH 7.2 of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of rabbit erythrocytes after 1 hr incubation at 25 °C.
Application
Lectin from Galanthus nivalis (snowdrop) has been used: to coat ELISA plates as reverse transcriptase inhibitorin ELISA for detection of envelope glycoproteins of the human and simian immunodeficiency viruses
Biochem/physiol Actions
GNL agglutinates rabbit erythrocytes; human erythrocytes are not agglutinated. GNL sugar specificity is directed to the nonreducing end of the terminal α-D-mannosyl residue of glycoconjugates.
Lectin from Galanthus nivalis (snowdrop) provide resistance against pests and are used in transgenic plants. Snowdrop lectin functions as a carrier to transport fused proteins to the hemolymph as it crosses gut epithelium of target species to exhibit insecticidal effects.
General description
Lectin from Galanthus nivalis (snowdrop) is a tetrameric, 12 kDa mannose binding lectin. It belongs to the family, Amaryllidacea. It is specific to α-1,3- or 1,6- linked D-mannose of carbohydrates. Snowdrop lectins are toxic to insects.
Packaging
5 mg in glass bottle
Preparation Note
Purified by affinity chromatography according to Kaku.
Ce produit répond aux critères suivants: