Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Browse additional application references in our FLAG^® Literature portal.

Features and Benefits

Utilizes highly specific ANTI-FLAG M2 affinity gel No preliminary steps or calibrations 3X FLAG^™ peptide provides efficient and gentle elution through direct competition

General description

Sigma’;s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.

Legal Information

3xFLAG is a trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Other Notes

All prodedures to be performed at 2-8°C.