Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Research CategoryCell Structure

The CHEMICON™ ECM Cell Adhesion Array kit is useful for assessing specific cell surface integrins, monitoring in vitro cell differentiation, or screening potential cell adhesion promoters/inhibitors.



For Research Use Only. Not for use in diagnostic procedures.

Components

ECM Array Plate: (PN: 90652) One 96-well plate with 12 strips. Each 8-well strip consists of 7 different human, ECM protein-coated wells (Collagen I, Collagen II, Collagen IV, Fibronectin, Laminin, Tenascin, Vitronectin) and one BSA-coated well (negative control). See the plate layout on Data sheet.Cell Stain Solution: (Part No. 90144) One bottle - 20 mL.Extraction Buffer: (Part No. 90145) One bottle - 20 mL.Assay Buffer: (Part No. 90601) One bottle - 100 mL.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Introduction:

Cell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. Scientists are continually examining the adhesion, migration, and invasion of many diverse cell types on various extracellular matrix (ECM) component proteins. The CHEMICON™ ECM Cell Adhesion Array Kit (Colorimetric) provides an ECM Array microtiter plate with a colorimetric detection format, allowing for large-scale screening and quantitative comparison of multiple samples/cell lines. The kit is designed as a rapid (1-2 hours), cost effective, efficient method for characterization of cell adhesion.

In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:

⋅ Alpha Integrin-Mediated Cell Adhesion Array Kit (Colorimetric) (ECM530)

⋅ Beta Integrin-Mediated Cell Adhesion Array Kit (Colorimetric) (ECM531)

⋅ Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit (Colorimetric) (ECM532)

⋅ Alpha Integrin-Mediated Cell Adhesion Array Kit (Fluorimetric) (ECM533)

⋅ Beta Integrin-Mediated Cell Adhesion Array Kit (Fluorimetric) (ECM534)

⋅ Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit (Fluorimetric) (ECM535)

⋅ ECM Cell Adhesion Array Kit (Fluorimetric) (ECM545)

⋅ QCM™ 8µm 96-well Chemotaxis Cell Migration Assay (ECM510)

⋅ QCM™ 5µm 96-well Chemotaxis Cell Migration Assay (ECM512)

⋅ QCM™ 3µm 96-well Chemotaxis Cell Migration Assay (ECM515)

⋅ QCM™ 96-well Cell Invasion Assay (ECM555)

⋅ QCM™ 96-well Collagen-Based Cell Invasion Assay (ECM 556)

⋅ 24-well Insert Cell Migration and Invasion assay systems

⋅ CytoMatrix™ Cell Adhesion strips (ECM protein coated)

⋅ QuantiMatrix™ ECM protein ELISA kits



Test Principle:

The CHEMICON™ ECM Cell Adhesion Array kit utilizes a colorimetric detection format, allowing large-scale screening and quantitative comparison of multiple samples. Each kit contains a 96-well, microtiter plate consisting of 12 X 8-well removable strips. Each well within a strip (7 wells total) is pre-coated with a different ECM protein (see table below) along with one BSA-coated well (negative control). Experimental cells are seeded onto the coated substrates, where adherent cells are captured. Subsequently, unbound cells are washed away, and the adherent cells are fixed and stained. After stain extraction, the relative cell attachment is determined using absorbance readings.



Additional information about the ECM components provided in the kit:

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

1 plate in 96 wells

96 wells

Storage and Stability

The ECM Array Plate can be stored at 2° to 8°C in the foil pouch up to its expiration date. Unused strips may be placed back in the pouch for storage. Ensure that the desiccant remains in the pouch, and that the pouch is securely closed. Keep the remaining kit components at 2° to 8°C.

Precautions:

⋅ Cell stain contains minor amounts of crystal violet, a toxic substance, which may cause cancer and heritable genetic damage. Handle with caution. Toxic by inhalation and if swallowed. Irritating to eyes, respiratory system and skin.

⋅ Extraction buffer contains alcohol. Avoid internal consumption.