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Application

SpecificityRed fluorescence detection reagents are often used with Texas Red^® filter.Application NotePrimary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink^® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells are required.Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink^® projectsView full Duolink^® product list

Features and Benefits

Multicolor Duolink^® proximity ligation assay (PLA^®) is an application of the Duolink^® technology that allows for endogenous, simultaneous detection of up to 4 protein interactions, post-translational modifications, or protein expression levels (including low abundant protein) in fixed cells and tissue samples. Follow the Duolink^® PLA Probemaker Guide for Multicolor Detection and the Duolink^® PLA Multicolor Detection protocol to use this product. Visualize and detect multiple proteins events in one experiment. No overexpression or genetic manipulation required High specificity (fewer false positives) Detection of low abundant proteins due to rolling circle amplification Relative quantification possible No special equipment needed Faster and more simple than FRET Increased accuracy compared to co-IP Publication-ready resultsComponentsDuolink^® PLA Multicolor Probemaker Kit - Red contains all the necessary reagents to perform the conjugations of Red Oligo A and Red Oligo B directly to primary antibodies. The resulting PLA probes (Red A and Red B) must be used together and cannot be paired with any other DuolinkThis product is comprised of the following: Red Oligo A DUO86010A Red Oligo B DUO86010B Conjugation Buffer DUO82033 Stop Reagent DUO82034 Storage Solution DUO82035See datasheet for more information.To perform a Multicolor Duolink^® PLA experiment primary antibodies (PLA, IHC, ICC or IF validated) that recognize protein targets of interest are required. Duolink^® PLA Multicolor Probemaker-generated probes (Red A/Red B, Green C/Green D, Orange F/Orange G, and/or FarRed H/FarRed I) are used in combination to perform the Multiplex experiment.The reagents in the Duolink^® PLA Multicolor Reagent Pack are required to perform the amplification and detection of the resulting PLA probes. Required additional reagents include Duolink^® Wash Buffers and Mounting Medium.

Legal Information

Duolink is a registered trademark of Sigma-Aldrich Co. LLC

Texas Red is a registered trademark of Life Technologies

PLA is a registered trademark of Sigma-Aldrich Co. LLC