Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Single day chromatin immunoprecipitation (ChIP) kit containing all necessary reagents to enable ChIP from low input amounts of chromatin obtained from either cells or tissues using magnetic A/G beads.

Components

10X Glycine;Nuclei Isolation Buffer;10X PBS;SCW Buffer (Sonication/ChIP/Wash);Magna ChIP Protein A/G Magnetic Beads;Low Stringency IP Wash Buffer;ChIP Elution Buffer;Protease Inhibitor Cocktail;Proteinase K Solution

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Robust ChIP from as few as 10,000 to as many as 1,000,000 cells Protocols and reagents for generation of chromatin from range of input sample type. Superior performance with variety of isotypes of either polyclonal or monoclonal antibodies Specialized buffer and bead formulations; lower backgrounds and higher fold enrichment. Single buffer system for sonication, chromatin IP, and wash Perform analysis of enrichment without additional purification after cross link reversal. Compatible with all commonly used downstream analysis applications– qPCR, next generation sequencing, microarray.

General description

The Magna ChIP HiSens kit provides a complete set of validated, quality controlled reagents, and a detailed protocol to enable ChIP from a wide range of input amounts of chromatin obtained from either cells or tissues. Our specialized blend of protein A/G blend of magnetic beads is specifically produced for chromatin immunoprecipiptation and enables the use of a broader range of antibodies than protein A or G alone eliminating the need to purchase different kits for different antibody isotypes. The SCW Buffer is unique to the Magna ChIP HiSens kit and enables the use of a single buffer for multiple steps of the ChIP process (sonication, chromatin immunoprecipitation, and wash). The ChIP elution buffer provided with the HiSens kit has been formulated to allow analysis of enrichment by qPCR without additional clean-up steps for more rapid results.

Legal Information

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

Kit capacity: 24 chromatin immunoprecipitation assays