Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Research CategoryEpigenetics & Nuclear Function

Components

2X Extraction BufferModification ReagentConversion BufferResuspension BufferEquilibration BufferBinding BufferWash Buffer IWash Buffer IIElution BufferProteinase KProteinase K Storage BufferDesalting ColumnsCollection Tubes

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Unlike other bisulfite modifications approaches that start with isolated genomic DNA, the CpGenome Direct Prep Bisulfite Modification Kit allows simple and reliable bisulfite conversion of DNA directly from a variety of starting materials, including cultured cells, blood, fresh tissue and fixed tissue samples.Key Features Bisulfite conversion directly from cells, tissues, blood and FFPE samples without DNA purification Sensitive bisulfite-conversion from as few as 10 cells or as low as 50 pg of input DNA Fast and simple, streamlined protocol for one-step bisulfite conversion In-column desulfonation allows recovery of DNA without additional precipitation steps for more consistent results Suitable for downstream analysis by methlylation specific PCR, restriction digestion, sequencing, microarray hybridization, etc.

Methylation of cytosines located 5′ to guanosine is known to have a profound effect on the expression of many eukaryotic genes. In normal cells methylation occurs predominantly in CG-poor regions, while CG-rich areas, called CpG-islands remain unmethylated. The exceptions are the extensive methylation of CpG islands associated with transcriptional inactivation of regulatory regions of imprinted genes and genes on the inactive X-chromosome of females. Aberrant methylation of normally unmethylated CpG islands has been documented as a relatively frequent event in immortalized and transformed cells and has been associated with transcriptional inactivation of defined tumor suppressor genes in human cancers. Hundreds of CpG islands are now known to exhibit the characteristic of hypermethylation in tumors resulting in identification of candidate genes that can be interrogated to determine extent of tumor specific transformation.

Linkage

Replaces: Replaces S7820 (CpGenome Universal DNA Modification Kit).