Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Suitable for transformation of any strain of yeast. Convenient, flexible and sensitive, positive transformants can be obtained with as little as 10 ng of DNA; the optimum efficiency is in the 0.1- 3 μg range.

Components

The Yeast Transformation Kit contains: Transformation Buffer; 100 ml; 100 mM lithium acetate, 10 mM Tris HCl, pH 7.6, and 1 mM EDTA Plate Buffer; 100 ml; 40% PEG, 100 mM lithium acetate, 10 mM Tris HCl, pH 7.5, 1 mM EDTA Deoxyribonucleic acid from salmon teste, 10 mg/ml; 2 x 1 ml Control Yeast Plasmid DNA pRS316 carrying the ura gene; 10 µg Yeast Synthetic Drop-out Medium Supplement Without Uracil; 1 g

Features and Benefits

Easy and ready-to-use Requires as little as 10 ng of plasmid DNA Flexibility for any strain of yeast Sufficient for over 100 standard transformations

General description

Sigma’;s Yeast Transformation Kit contains all necessary reagents and controls for efficient transformation of yeast by the lithium acetate method.

Principle

Transformation with a plasmid complementing the mutated gene enables the transformant to grow on medium lacking the required component. Yeast cells are made competent for transformation by incubation in a buffered lithium acetate solution. Transformation is then carried out by incubating the cells together with transforming DNA and carrier DNA in a solution containing polyethylene glycol (PEG).