Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Spectrum^® Plant Total RNA Kit has been used to isolate RNA from ground fiber tissue of cultured cotton fiber, from neem tissues and from Sarracenia, an insectivorous plant.

Features and Benefits

Yields up to 60 µg of pure concentrated RNA per prep Efficient protocol allows for RNA purification in 30 min or less Specially designed for research with difficult plant tissues

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry.

The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 µg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 µg. After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants. Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.

Legal Information

Spectrum is a trademark of Sigma-Aldrich Co. LLC

SabreLite is a registered trademark of Pelican Products, Inc.