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Application

The purified total RNA is ready for use in any enzymatic downstream application.

Features and Benefits

Unique plate design offers low risk of cross-contamination RNA can be used for any kind of downstream enzymatic reaction Processing possible under with a vacuum or centrifuge Suitable for manual and automated processing

General description

GenElute 96 Well Total RNA Purification Kit allows for the high-throughput purification of total RNA from cells or tissue. The kit utilizes the advantages of silica membrane technology and eliminates the use of hazardous organic compounds such as phenol and chloroform.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Other Notes

Application notes: Method of purification: Silica-membrane technology Compatible with Manual or automated, vacuum or centrifugation Efficient recovery of > 200 nt Typical yield 20 µg (2 x 106 HeLa cells), 20 µg (20 mg mouse liver) Typical concentration 50–200 ng/µL Elution volume 50–130 µL Preparation time 70 min/plate Binding capacity 100 µg RNA ratio 28S/18S approx. 2.1 A₂₆₀/A₂₈₀ 1.90–2.10 Typical RIN (RNA > 9 (cells)

Principle

Sigma′s GenElute 96 Well Total RNA Purification Kit provides a simple and convenient way to isolate total RNA from cells or tissue. The cells or tissue are lysed in a solution containing large amounts of chaotropic salt which immediately inactivates RNases that are present in almost all biological materials. The addition of a Wash Buffer provides the necessary binding conditions for the RNA to be adsorbed to the silica membrane. The contaminating DNA which may be bound to the silica membrane is removed by directly applying RNase-free DNase. The remaining salts, proteins, and other cellular debris are removed by additional washing steps. The final step is to elute the purified RNA in the RNAase-free water that is supplied in the kit.