Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

KAPA Taq PCR Kit with dNTPs has been used in:High throughput PCRAmplification of low copy DNA templatesMultiplex PCRSpecific amplification of complex templatesRT-PCRQuantitative polymerase chain reaction (qPCR)

Biochem/physiol Actions

KAPA Taq DNA Polymerase is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. KAPA Taq and KAPA Taq HotStart^® DNA Polymerase have 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′ → 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 10⁵ nucleotides incorporated. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.

Features and Benefits

High performance Improved sensitivity, specificity, and yields. Novel buffer formulation facilitates specific primer annealing, leading to higher yield of specific product.Quick Notes: KAPA Taq HotStart^® DNA Polymerase can replace any commercial hotstart Taq DNA polymerase in an existing protocol. The final MgCl₂ concentration and annealing temperature may need to be optimized to account for differences in formulation. The KAPA Taq HotStart Buffer is a uniquelyformulated buffer offering improved specificity and sensitivity, and improved amplification of GC- and AT-rich templates. The KAPA Taq HotStart Buffer does not contain MgCl₂; MgCl₂ (25 mM) is supplied separately to allow greater flexibility during reaction setup. The KAPA Taq HotStart PCR Kit is suitable for the amplification of fragments up to 3.5 kb from genomic DNA or 5 kb from less complex targets.

General description

KAPA Taq is supplied in a 2X ReadyMix^™ format containing all the components required for PCR except primers and template. Simply use PCR-grade water to make up the required reaction volume. KAPA Taq Buffer A (and KAPA Taq Buffer with dye) are standard Tris-ammonium sulfate-based buffers, while KAPA Taq Buffer B is a Tris-potassium chloride buffer, which allows convenient direct analysis of the PCR product by agarose gel electrophoresis after cycling. All KAPA Taq Buffers are 10X buffers, containing 15 mM MgCl2 (1.5 mM at 1X). KAPA Taq DNA Polymerase may be used in combination with any standard Taq buffer with a pH of 8.3 or higher.

Legal Information

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

HOTSTART is a registered trademark of Molecular BioProducts, Inc.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Preparation Note

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long term storage.

Quality

Each batch of KAPA Taq HotStart DNA Polymerase is confirmed to contain ﹤2% contaminating protein (Agilent Protein 230 Assay). KAPA Taq HotStart PCR kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.