Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

KiCqStart^® SYBR^® Green qPCR ReadyMix^™ has been used to perform quantitative real-time PCR (qPCR) for the amplification and quantification of genomic DNA extracted from human gut bacterium Prevotella copri.

PCR applications: Gene expression DNA quantification CHiP

Components

2X reaction buffer containing optimized concentrations of MgCl₂, dNTPs (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, and stabilizerspackaging:250 reactions* = 2 X 1.25 mL tubes1250 reactions* = 10 X 1.25 mL tubes5000 reactions* = 1 X 50 mL tube*number of reactions based on a 20uL volume

Features and Benefits

Assay results in as little as 33 minutes Highly efficient and sensitive real-time PCR results Little/no optimization required

General description

KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use master mix that contains all components, except primers and template, for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR.Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step.

Legal Information

SYBR is a registered trademark of Life Technologies

Chromo4 is a trademark of Bio-Rad Laboratories, Inc.

Mastercycler is a registered trademark of Eppendorf AG

MiniOpticon is a trademark of Bio-Rad Laboratories, Inc.

Opticon is a trademark of Bio-Rad Laboratories, Inc.

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

CFX384 is a trademark of Bio-Rad Laboratories, Inc.

SmartCycler is a registered trademark of Cepheid, Inc.

Eppendorf is a registered trademark of Eppendorf AG

LightCycler is a registered trademark of Roche

CFX96 is a trademark of Bio-Rad Laboratories, Inc.

Rotor-Gene is a registered trademark of Qiagen GmbH

Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.

KiCqStart is a registered trademark of Qiagen Beverly Inc.

Other Notes

Instrument Compatibility:Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix does not contain an internal reference dye. Compatible instruments include: Bio-Rad^® CFX384^™ Bio-Rad CFX96^™ Bio-Rad MiniOpticon^™ Bio-Rad/MJ Chromo4^™ Bio-Rad/MJ Opticon 2 Bio-Rad/MJ Opticon^™ Cepheid SmartCycler^® Eppendorf Mastercycler^® ep realplex Eppendorf Mastercycler ep realplex2 s Illumina Eco qPCR Qiagen/Corbett Rotor-Gene^® 3000 Qiagen/Corbett Rotor-Gene 6000 Qiagen/Corbett Rotor-Gene Q Roche LightCycler^® 480

Storage Conditions:KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.