Components

BL21(DE3) pLysE chemically competent cells pUC 19 transformation control DNA recovery medium for expression

Features and Benefits

a transformation efficiency of >1 × 10⁷ cfu/µg. economical prices offering superior value for everyday protein expression work.

General description

The BL21(DE3) pLysE Chemically Competent Cells produce T7 lysozyme to repress low-level transcription from the T7 promoter prior to IPTG induction, helping to stabilize inserts coding for deleterious products. These cells have a higher level of repression by T7 lysozyme, offering the greatest control over expression of inserts.GenotypeF – ompT hsdSB (rB- mB-) gal dcm (DE3)