Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Binds with high specificity to single-stranded DNA. Can be useful in enhancing the specificity of PCR and in enabling the sequencing of problematic DNA templates.

Single-strand Binding Protein from Escherichia coli has been used in in vitro RNA/Single-Strand Binding Protein (SSB) complex formation assays and in protein purification to determine integrity and functionality of the integron attC sites.

Biochem/physiol Actions

Single-strand Binding Protein (SSBP) exhibits high affinity towards single stranded DNA important for DNA metabolic processes. Single-stranded DNA-binding protein hSSB1 is critical for genomic stability and assists the delivery of primers during the formation of lagging standard. It also prevents the formation of inhibitory secondary structures in the exposed lagging-strand of DNA during replication. SSBP mediates the recruitment of other proteins to their sites of function on DNA.

General description

Single-strand Binding Protein is a homotetramer, found in E. coli during the event of DNA metabolism.

Physical form

Solution in 20 mM Tris-HCl, pH 8.0, 0.5 M NaCl, 0.1 mM EDTA, 0.1 mM DTT, 50% glycerol