Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

REDTaq^® Genomic DNA Polymerase has been used as a component of the polymerase chain reaction (PCR) mix for PCR amplification. It has also been used as a component of the preamplification mix for PCR in amplified fragment length polymorphism (AFLP) analysis of Fusarium oxysporum sp. isolates.

Components

Includes 10× PCR reaction buffer without MgCl₂ and a separate tube of 25 mM MgCl₂

Features and Benefits

Enhanced amplification on genomic and difficult DNA templates Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications Quick recognition and confirmation of appropriate mixing No loading buffers or tracking dyes necessary. Sample can be taken directly from reaction and loaded onto an agarose gel The samples can be re-amplified as in nested PCR The red dye migrates faster than bromophenol blue Greater consistency across reactions due to proper mixing

General description

REDTaq^® Genomic DNA Polymerase is a unique blend of Taq DNA Polymerase with an inert red dye. This special formulation is designed to provide enhanced amplification of more complex or genomic templates. REDTaq Genomic DNA Polymerase is highly sensitive, produces increased yields and is capable of generating longer product lengths. It has all the advantages of REDTaq DNA polymerase, such as easy visualization of enzyme addition and complete reaction mixing, and direct loading to an agarose gel. The inert red dye does not effect automated or manual sequencing, restriction digestions or other downstream applications. The dye can easily removed by any standard purification method.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

REDTaq is a registered trademark of Sigma-Aldrich Co. LLC

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min. at 74°C.