Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

KAPA Taq PCR Kit has been used in:High throughput PCRAmplification of low copy DNA templatesMultiplex PCRSpecific amplification of complex templatesRT-PCRrandom amplified polymorphic DNA polymerase chain reaction (RAPD-PCR)Polymerase chain reaction (PCR) Genotyping

Biochem/physiol Actions

KAPA Taq PCR Kit, which contains KAPA Taq DNA Polymerase, is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. KAPA Taq and KAPA Taq HotStart^® DNA Polymerase have 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′ → 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 10⁵ nucleotides incorporated. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.

Features and Benefits

High performance: Improved sensitivity, specificity, and yields Novel buffer formulation facilitates specific primer annealing, leading to higher yield of specific productQuick Notes: KAPA Taq DNA Polymerase can replace any commercial Taq DNA polymerase in an existing protocol. The final MgCl₂ concentration may need to be optimized to account for differences in buffer formulation. KAPA Taq Buffers contain MgCl₂ at a final concentration of 1.5 mM. Buffer A is recommended as first approach and for applications requiring high yields. Buffer B is recommended for applications where high sensitivity is required (e.g. when the template is limiting). Both buffers may be evaluated to determine the buffer most suitable for a specific application. The KAPA Taq PCR system is suitable for the amplification of fragments up to 3.5 kb from genomic DNA or 5 kb from less complex targets.

Legal Information

HOTSTART is a registered trademark of Molecular BioProducts, Inc.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Preparation Note

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C forshort-term use (up to 1 month). Return to -20°C for long term storage.

Quality

Each batch of KAPA Taq DNA Polymerase is confirmed to contain ﹤2% contaminating protein (Agilent Protein 230Assay). KAPA Taq Ready Mixes are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.