Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

For research use only. Not for use in diagnostic procedures.The MIA ELISA is an ideal application in research studies determining the melanoma inhibitory activity protein in serum and plasma.The MIA ELISA is specially developed for the quantitative in vitro determination of MIA protein in serum and plasma within streptavidin-coated microplates (MPs). It has a wide applictaion in MIA (melanoma inhibitory activity) ELISA assays.

General description

One-step immunoreaction—photometric enzyme immunoassay for the quantitative in vitro determination of human Melanoma Inhibitory Activity (MIA) in streptavidin-coated microplates.Standards: Six standards, ranging from 0 to 50 ng/ml MIA protein, are provided with lot-specific data.

Immunogen

Detailed epitope structure is not known. The epitopes recognized by the antibodies are conformation epitopes, no linear structures. This is why the abs are not suitable for Western (denaturation destroys epitopes). The two antibodies recognize different epitopes (non-overlapping). The antibodies recognize non-overlapping epitopes, which are conformational epitopes (no linear sequence is recognized but rather parts of 3D-conformation). Exact epitopes have not been determined.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Packaging

1 kit containing 9 components.

Preparation Note

Working solution: Beside the ready-to-use solutions supplied with this kit, you will need to prepare the following working solution:Anti-MIA-Biotin: Reconstitute the lyophilizate in 0.7 ml double-dist. water for 10 minutes at 15 to 25 °C and mix thoroughly. Do not vortex.Result: A clear, colorless solution. Anti-MIA-Peroxidase: Reconstitute the lyophilizate in 0.7 ml double-dist. water for 10 minutes at 15 to 25 °C, and mix thoroughly. Do not vortex.Result: A clear, slightly yellowish solution. MIA Standard: Reconstitute the lyophilizate in 0.5 ml double-dist. water for 10 minutes at15 to 25 °C, and mix thoroughly.Do not vortex.Result: A clear, colorless solution. Concentration indicated on the label. MIA Control Serum: Reconstitute the lyophilizate in 0.3 ml double-dist. water for 10 minutes at 15 to 25 °C, and mix thoroughly. Do not vortex.Result: A Slightly opalescent and yellowish solution. Concentration indicated on the label. Washing Buffer : Dissolve one tablet in 2 l of double-dist. water.Result: A clear, colorless solution. Immunoreagent: For 8 wells (1.8 ml): Add 50 µl reconstituted anti-MIA-Peroxidase (solution 2) to 1.7 ml Incubation Buffer, and mix well. Then add 50 µl of anti-MIA-biotin(solution 1), and mix thoroughly. Do not vortex.Result: A clear, colorless solution.All lyophilizates should be clear solutions after reconstitution. Any particles in the reconstituted solution should be considered as evidence of deterioration. Precipitates or cloudiness in the reagent solutions should also be considered as indications of deterioration.Roche recommends using only double distilled water for the reconstitution of lyophylizates.Storage conditions (working solution): Anti-MIA-Biotin (vial 1):6 months at -15 to -25 °C or ≤ -60 °C when aliquoted and shock-frozenAnti-MIA-Peroxidase (vial 2):6 months at -15 to -25 °C or ≤ -60 °C when aliquoted and shock-frozenMIA-Standard (vial 3):1 day at 2 to 8 °C or 6 months at -15 to -25 °C or ≤ -60 °C when aliquoted and shock-frozenMIA-Control Serum (vial 4):1 day at 2 to 8 °C or 6 months at -15 to -25 °C or ≤ -60 °C when aliquoted and shock-frozenWashing Buffer (vial 6): 1month at 2 to 8 °CImmunoreagent: 8 hours at 2 to 8 °C

Specificity

The MIA ELISA detects natural and recombinant human melanoma inhibitory activity protein. No cross-reaction with other serum components has been found.