Application

The Cell Proliferation ELISA, BrdU (chemiluminescent) belongs to the second, improved generation of kits for measuring DNA synthesis. It is a precise, fast, and simple nonradioactive alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in replicating (cycling) cells. Thus, the Cell Proliferation ELISA can be used in many different in vitro cell systems. For example:Detection and quantification of cell proliferation induced by growth factors and cytokinesDetermination of the inhibitory or stimulatory effects of various compounds on cell proliferation in environmental and biomedical research, and in the food, cosmetic, and pharmaceutical industriesMeasurement of the immunoreactivity of lymphocytes, stimulated by mitogens or antigensAnalysis of the chemosensitivity of tumor cells to different cytostatic drugs in medical research

Cell Proliferation ELISA, BrdU (chemiluminescent) has been used: for bromodeoxyuridine (BrdU) proliferation assay in ReN and primary astrocytes to test the effect on imatinib on the proliferation of neuroblastoma cells using BrdU incorporation assayto check the effect of palbociclibon cell cycle inhibition using BrdU incorporation in pancreatic patient-derived primary cell lines

General description

Chemiluminescent immunoassay for the quantification of cell proliferation, based on the measurement of BrdU incorporation during DNA synthesis:  a nonradioactive alternative to the [³H]-thymidine incorporation assay.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Packaging

1 kit containing 7 components.

Specificity

The antibody conjugate reacts with the thymidine analogue 5-bromo-2′-deoxyuridine (BrdU) and with BrdU incorporated into DNA. For binding to BrdU incorporated into the DNA, the BrdU-labeled DNA has to be denatured. The antibody does not cross-react with any endogenous cellular components such as thymidine, uridine, or DNA.