Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

The kit is used for the rapid identification of class, subclass, and light-chain types of mouse monoclonal antibodies (immunoglobulins).

Features and Benefits

Reliable: Yields comparable results to the standard ELISA assay Fast: 5 minutes from start to finish Easy: Only 3 steps Economical: No equipment or additional reagents required Highly specific: No crossreactivity with FBS

General description

IsoStrip Mouse Monoclonal Antibody Isotyping Kit is a simple, three-step kit for the rapid characterization of mouse monoclonal antibodies.Contents Development Tubes, contain lyophilized latex beads, precoated with anti-mouse-Ig antibodies Isotyping Strips, precoated with subclass- and light-chain-specific anti-mouse-Ig antibodies

Legal Information

IsoStrip is a trademark of Roche

Other Notes

For life science research only. Not for use in diagnostic procedures.

Packaging

1 kit containing 2 components.

Preparation Note

Working solution: Dilute Sample Remove the desired number of isotyping strips from the canister. Remove the caps from an equal number of development tubes.The tubes may be labeled with a pencil or felt-tipped lab marker for easy identification. Dilute a sample containing the mouse monoclonal antibody in 1% BSA/ phosphate-buffered saline (PBS), pH 7.2 to 7.6.Culture supernatant samples should be diluted 1:10 to 1:100. Ascites samples should be diluted 1:20,000. These are recommended dilutions and may vary depending on the concentration of antibody in your sample. In our experience, a monoclonal antibodyconcentration of 0.1 to 1 g/ml of diluted sample gives the best results. 150 ml of this diluted sample will be added to the development tube.

Specifications

Assay time: 5 minutesSample material: Hybridoma supernatant, purified mouse monoclonal antibodies, ascites

Specificity

Anti-mouse antibodies, bound to the latex beads, will react with any mouse monoclonal antibody regardless of its isotype.Goat anti-mouse antibodies, immobilized to the strip, specifically bind to each of the common mouse antibody isotypes (IgG₁, IgG_2a, IgG_2b, IgG₃, IgM, and IgA), and to the light chains.Specificity for all specific antibodies beside IgML at 1.0 µg/ml: ≥ 2IgM Lambda, at 1.0 µg/ml: ≥ 1