Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Laminin has been demonstrated to promote the attachment and growth of a variety of cells; used for the coating of culture dishes. It has been used for cell adhesion assay. It is recommended for use as a cell culture substratum at 1-2μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.

Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 µg/cm². The optimal concentration does depend on cell type as well as the application or research objectives.

General description

Laminin is purified as laminin-nidogen complex from mouse Engelbreth-Holm-Swarm (EHS) sarcoma. Laminin is a heterotrimer made from α, β and γ chain subunits. It is a glycoprotein which is required for the structural scaffolding of basement membranes. Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells.Biological activity: Tested for the promotion of adherence of HT-1080 cells.Species specificity: Active on most mammalian cells.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Solution, filtered through 0.2 ?m pore size membrane

Solution, 0.5 mg/ml laminin in 0.15 M NaCl, 2 mM EDTA, 0.05 M Tris-HCl, pH 7.4, sterile

Preparation Note

Working concentration: 2 to 5 µg/cm²2 to 5 µg/cm² for the coating of cell culture vesselsStorage conditions (working solution): 2 to 8 °CThe undiluted solution is stable at 2 to 8 °C for at least 3 months.The solution should be thawed carefully at 2 to 8 °C or on ice.

Quality

Purity: ≥90% (SDS-PAGE) (as laminin nidogen-complex, 1:1)

Sequence

Mouse laminin is composed of three polypeptide chains (A: 400 kD, B1: 230 kD and B2: 220 kD), connected by disulphide bridges, and is glycosylated.