Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

Absorption: The spectral data of Biotin-16-dUTP (at pH 7.3):Emax₂₄₁: E = 10.700;Emax₂₉₂: E = 7.000;Emax₂₆₅: E = 4.400;A_292/265 : 1.6.

Application

For nonradioactive DNA labeling such as random priming, PCR labeling, or nick translation.Biotin-16-dUTP is used as asubstrate for: Terminal TransferaseDNA polymerase I (holoenzyme and Klenow fragment)Taq DNA polymeraseReverse transcriptase (e.g., Transcriptor)Biotin-16-dUTP replaces dTTP in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% Biotin-16-dUTP and 65% dTTP, as well as in PCR.The nucleotide also serves as a substrate for Terminal Transferase in 3′-end labeling.Biotin-labeled DNA can be detected with: Streptavidin-alkaline phosphatase conjugate and a chemiluminescent substrate (CSPD, CDP-Star) or a color substrateBiotin Luminescence Detection Kit

Biochem/physiol Actions

Biotin influences gene regulation and chromatin structure by associating with lysine residues in histones. It influences the actions of adaptive immune T and natural killer cells. Deficiency of biotin in mice has indicated enhanced inflammation. Studies have also indicated heightened inflammatory response of human dendritic cells in conditions of biotin deficiency.

General description

Biotin is a water-soluble vitamin which functions as a coenzyme for carboxylases.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Quality

Typical analysis: At least 85% deoxynucleoside triphosphate (Biotin-16-dUTP) (HPLC, area%).Notes: For your convenience, biotin-dUTP is also available in convenient labeling mixes (single solution with conventional dNTPs and biotin-16-dUTP), and technique-specific mixes (nucleotides, buffers, and enzymes in a single vial).