Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Alkaline phosphatase can be coupled to other proteins via its amino groups. The enzyme solution can be used directly for conjugation without prior dialysis.

General description

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)Amino groups: 8-16 moles/mole enzyme

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Solution, ready-to-use, 10mg/ml

Preparation Note

Storage conditions (working solution): The diluted enzyme (1:10 in 3 M NaCl) will give a stability of 6 months at 4 °C.1:10 dilution in water will give stability for 1 week at 4°C.Do not freeze the diluted or undiluted enzyme, because freezing will destroy enzyme activity.

Quality

Purity: single peak (HPLC)

Sequence

Purification procedureAP from intestine is a dimeric glycoprotein, it is not known, whether the carbohydrate part is separated during purification procedure. Content of amino groupsIn the literature the carbohydrate content is 2 to 20 percent so one should find approx. 70 amino groups/mol.In addition one researcher determined the amino groups and found 50 to 60 lysins/mol.-SH groups in APNo information in literature about -SH groups in AP.Carbohydrate structureResults of investigation of the carbohydrate structure of several lots of alkaline phosphatase are available.NH₂- and carbohydrate estimationResults of NH₂- and carbohydrate estimation are available.

Storage and Stability

Store at 2 to 8 °C. (Do not freeze)

Unit Definition

Unit Conversion: Approx. 4.35 U (EIA grade AP) [37 °C, 4-NPP as substrate, diethanolamine as buffer, pH 9.8] = 1 U [+25 °C, 4-NPP as substrate, glycine as buffer, pH 10.5]1.0 U [+37 °C, QC assay conditions] = 0.73 U [+30 °C, QC assay conditions] = 0.48 U [+25 °C, QC assay conditions]