Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

It was used in enzymatic assay to measure citrate concentration using microtiter plates.

General description

Citrate lyase is considered as a substrate- induced enzyme which helps in catalyzing reversible aldol cleavage of citrate to oxaloacetate and acetate. The enzyme is adaptive to anaerobic dissimilation of citrate.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Lyophilizate, stabilized with BSA, sucrose, MgSO₄ and EDTA. Note: A solution in double-distilled water has a pH-value of approximately 7.0. The enzyme can be relyophilized.

Preparation Note

Activator: Mg²⁺ (K_m = 3.0 mM, pH 7.4, 25 °C); other divalent metal ions (Mn²⁺, Fe²⁺, Zn²⁺)

Quality

Contaminants: ﹤0.05% ICDH (NAD specific) and “NADH oxidase”, each