Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

RNase has been used in DNA ladder assay.

RNase has been used in analysis of DNA fragmentation using agarose gel electrophoresis. It has been used during flow cytometry analysis.

General description

Human chromosome 14q11.2 shows the presence of all human RNases. Only eight (RNase 1-8) of them were true called as canonical RNases. And the rest nine to thirteen are called as noncanonical RNases. Human RNase 1 is called as HP-RNase or human pancreatic RNase. Human RNase 2 is also called as EDN (eosinophils derived neurotoxin) that is isolated from eosinophils.

Crude mixture of RNases.Dry powder (prepared from crystallized ribonuclease).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Activator: PK requires Mg²⁺ (or Mn²⁺, Co²⁺) and K⁺ (or NH⁴⁺, Rb⁺) for full activity.Working solution: It is recommended to dissolve the RNase (1 mg/ml) in water or TE buffer, pH 7.5.

Specificity

Heat inactivation: 1 mM EDTA

Unit Definition

One unit is the enzyme activity that causes a decrease in absorbance of A₀ to A₁ within 1 minute under the assay conditions; A₀ to A₁ corresponds to the total conversion, A₁ being the final absorbance.