Non disponible en dehors du Royaume-Uni et de l'Irlande
Application
Polyadenylic acid (Poly(A)) to inhibit Exo1′s exonuclease activity. It has also been used as a carrier to resuspend lyophilized gBlocks.
Poly(A) has been used for droplet digital PCR (ddPCR) assay.
Biochem/physiol Actions
Polyadenylic acid (Poly(A)) tails present at 3′ end are produced in the cell nucleus. It contains ~250 nucleotides in mammalian cells.Poly(A) regulates mRNA decay and the initiation of translation. Cytoplasmic poly(A) extension modulates translation.
General description
Poly(A) is used as a carrier for quantitative precipitation of DNA and RNA.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Physical form
Lyophilizate, potassium salt
Preparation Note
Working concentration: 0.5 mg/mlA concentration of 0.5 mg/ml is recommended.Working solution: 0.5 mg/ml is recommended.
Quality
Typical analysis:2.3µmol/mg Poly(A) (from absorbance) in relation to one mononucleotide unit. Chromatographically homogeneous.Absorbance determination A250/A260, A280/A260, A290/A260
Sequence
Chain Length 2.100 to 10.000 nucleotides
Unit Definition
1 A260 unit corresponds to 40 µg ssRNA.
Ce produit répond aux critères suivants: