Bundle including MagBio HighPrep™ PCR clean-up system CE-IVDR and MagQuant Plus DNA V2 kit.

HighPrep™ PCR is a paramagnetic beads-based post PCR clean-up reagent designed for efficient purification of amplicons and tight size-specific selection of DNA fragments. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapter ligation, microarrays, and so on. This protocol can be used for manual procedures as well as in automation.

The MagQuant Plus DNA Kit V2 utilises paramagnetic bead-based technology for genomic DNA quantitation and concentration normalisation. The DNA normalisation occurs during the purification process. Standard methods of DNA quantitation require the use of a standard curve derived from DNA samples of known concentrations; processing DNA samples from various sources would require extensive work and reagent use. MagQuant Plus DNA V2‘s proprietary technology allows to obtain a given DNA quantitation and required concentration of DNA samples regardless of the source by adding a specific number of beads to the DNA sample.