Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

Yeast produce green, opaque large colonies. Mold colonies are green and filamentous

Target Microorganisms: Yeast and mold (selection by pH)

QC organismsS. cerevisiae (ATCC 9763)A. niger (ATCC 16404)

Application

m-Green yeast and mold Broth is used for the testing soft drinks and other beverages like beer, bottled water, cider, soft drink, sport drink and wine for growth of yeast and mold.

Culture Medium

m-Green Yeast and Mold

General description

m-Green Yeast and Mold Broth is a relatively more complex formula compared to other media used for isolation of fungi and yeast.{10} This formulation is rich in nutrient which allows excellent fungal growth. Bacterial growth is inhibited by an acidic pH. Enzymatic digest of casein and enzymatic digest of animal tissue provide nitrogen, carbon, and amino acids in m-Green yeast and mold Broth. Yeast extract is the vitamin source. Dextrose is an energy source for metabolism of fungi. Potassium phosphate is a buffering agent. Magnesium sulphate, Thiamine, and Diastase (a mixture containing amylolytic (starch) enzymes) provide essential ions, minerals, and nutrients. Bromocresol Green is the pH indicator, facilitating visualization and counting of fungal colonies. The colonies are green due to the diffusion of bromocresol green into the colonies (alkaline reaction). Acidic end products from the colonies diffuse into the medium, further reducing the pH and causing the dye to turn yellow (acid reaction). Yeast produces green, opaque large colonies. Mold colonies are green and filamentous.

Legal Information

MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany

MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

50×2 mL in ampoules

2 mL plastic ampoules

Specifications

Incubation Temperature: 28–32 °C

Incubation Time: 48–72 hrs