ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Each system includes the following reagents, sufficient for labeling 5 to 10µg nucleic acid and detecting 2000 to 4000cm² of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL Detection Reagents, and ECL Gold Hybridization Buffer.