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Features and Benefits

Unique selectivity Similar retention to C18 (sometimes requires stronger mobile phase) Excellent peak shape Stable, low-bleed LC-MS separations Scalable separations from 3 to 10µm particle sizes

General description

Guidelines for transferring a C18 method to Discovery® HS F5:Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log P_o/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.

The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.

Legal Information

Discovery is a registered trademark of Sigma-Aldrich Co. LLC

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