Découverte BIO Wide Pore C5 colonne HPLC, taille des particules 10 mum, l x i.d. 25 cm x 10 mm

Code: 567235-u D2-231

Non disponible en dehors du Royaume-Uni et de l'Irlande

General description

Discovery BIO Wide Pore C5 was designed for the efficient and reliable separation of proteins and peptides, especially hydrophobic peptides, by RP- HPLC. ...


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$1,812.50 EACH
$2,175.00 inc. VAT

Non disponible en dehors du Royaume-Uni et de l'Irlande

General description

Discovery BIO Wide Pore C5 was designed for the efficient and reliable separation of proteins and peptides, especially hydrophobic peptides, by RP- HPLC. Long-chain phases, like C8 or C18, are often too hydrophobic for proteins and can cause excessively long retention time or even irreversible binding to the column. For this reason short-chain phases, typically C3 or C4, are often used for RP-HPLC of proteins. However, these short-chain phases are susceptible to hydrolysis, resulting in short column lifetime, especially at low pH. The Discovery BIO Wide Pore C5 gives elution order similar to a conventional C4, yet has enhanced pH stability for longer column lifetime. Generally, higher efficiency separations are achievable on the Discovery BIO Wide Pore C5 because of the improvements we have made to the silica and bonded-phase chemistry.

Legal Information

Discovery is a registered trademark of Sigma-Aldrich Co. LLC

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extent of labeling3.5% Carbon loading
featureendcapped
L × I.D.25 cm × 10 mm
manufacturer/tradenameDiscovery®
materialstainless steel column
matrix active groupC5 (pentyl) phase
matrixfully porous particle, silica particle platform
operating pH2-8
packaging1 ea of
parameter400 bar pressure (5801 psi), 0-70 °C temperature
particle size10 µm
pore size300 Å
product lineDiscovery®
Quality Level100
separation techniquereversed phase
surface area100 m2/g
surface coverage4.5 µmol/m2
technique(s)HPLC: suitable, LC/MS: suitable
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