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Analysis Note

Protein determined by E1%/280

Application

Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies .

Biochem/physiol Actions

Ribonuclease T1 (RNase T1) from Aspergillus oryzae is an endoribonuclease that hydrolyzes after G residues. Cleavage occurs between the 3’;-phosphate group of a guanidine ribonucleotide and 5’;-hydroxyl of the adjacent nucleotide. The initial product is a 2’;:3’; cyclic phosphate nucleoside that is hydrolyzed to the corresponding 3’;-nucleoside phosphate. It differs from Pancreatic RNase in that it attacks the guanine sites specifically to yield 3′-GMP and oligonucleotides with a 3′-GMP terminal group.

Physical form

Suspension in 2.8 M (NH₄)₂SO₄ solution

Unit Definition

One unit will produce acid soluble oligonucleotides equivalent to a δA₂₆₀ of 1.0 in 15 min at pH 7.5 at 37°C, in a reaction volume of 1.0 mL. Substrate: Yeast RNA.